Seeing if one could watch the entire clip without looking away became a "rite of passage" in certain online subcultures.

The video is a brief, low-quality clip (hence the .wmv Windows Media Video format) that depicts a graphic and unsettling medical procedure or injury. Specifically:

The title "thick.100" is widely believed to refer to the consistency of the biological matter shown in the wound or the "thickness" of the gauze and packing being used to treat it.